In this paper, a novel fluorescent aptasensing strategy for protein assay has been developed based on target-triggered hybridization chain reaction (HCR) and graphene oxide (GO -based selective fluorescence quenchinGO -based
An ultrasensitive electrochemical detection of target DNA was developed based on target-triggered hairpin assembly and exonuclease III (Exo III -assisted recyclIII -assisted amplification strecycling
A novel fluorescence biosensing strategy for ultrasensitive and specific detection of pathogenic bacteria based on target-triggered enzymatic repairing amplification (ERA) has been developed.
Therefore, the proposed aptasensing strategy based on target-triggered hybridization chain reaction amplification should have wide applications in the diagnosis of genetic diseases due to its simplicity, low cost, and high sensitivity at extremely low target concentrations.
Herein, we propose a colorimetric method for protein assays based on target-triggered activation of aptazyme, which may offer simple, rapid and cost-effective detection of the target protein.
We herein describe a simple, enzyme-free, and label-free strategy for the detection of miRNA based on target-triggered catalytic hairpin assembly (CHA) and fluorescence enhancement of DNA-silver nanoclusters (DNA-AgNCs).
This work designs a new label-free aptasensor for the colorimetric determination of small molecules (adenosine 5′-triphosphate, ATP) by using visible gold nanoparticles as the signal-generation tags, based on target-triggered hybridization chain reaction (HCR) between two hairpin DNA probes.
Based on target-catalyzed hairpin assembly, target miR-122 could trigger the hybridization of capture probes and signal probes to further be released to initiate the next reaction process resulted in numerous tracer indicators anchored onto the sensing interfaces.
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Our approach is based on targeting of a quenched fluorescent pHLIP-FIRE construct to acidic tumors and activation of the fluorescence by translocating the cargo dye into cells, where the reducing power of the cytosol triggers the enhanced fluorescence.
