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Based on substrate depletion, K48 chains were less effectively used than K63 chains, and whereas multiple units of K48- and K63-di-ubiquitin were attached, HisUb PCNA was modified by no more than a single unit of K48-tetra-ubiquitin.
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Yield coefficient of cell based on substrate utilization (g/g).
In these cases, however, substrate selectivity is not a general property and will vary based on substrate levels and substrate-dependent kinetic interaction rates.
In the UAE, we have embarked on a program to transform our economy from one based on resource depletion to one based on resource enrichment.
Based on the depletion of the mono-ubiquitylated substrate, we found that stimulation of the reaction required both DNA and RFC, suggesting that mono-ubiquitylated PCNA is a better substrate for Ubc13 Mms2-Rad5 when residing on DNA than free in solUbc13 Mms2-Rad5
NEU4 was tested with GM3 and 4MU-NeuAc substrates, based on its substrate preference.
Liu, A. et al. High-speed optical modulation based on carrier depletion in a silicon waveguide.
Based on the depletion efficiency of the Rac1-specific shRNAs (Fig. 6A,B), we chose shRac1-1 as the Rac1 interfering sequence for the following experiments.
We compare the resulting gain changes to a model based on neurotransmitter depletion [17].
Based on a depletion and reconstitution strategy, we could identify an important role for classical monocytes.
Many substrates have been identified for each exo-RNase [ 4], but no endogenous substrates have been assignment of to these enzymes based on global substrate profiling.
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