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The Michaelis-Menten function is often used in studies on enzyme kinetics that describe the rate of enzymatic reactions based on substrate concentration [ 30].
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Yield coefficient of cell based on substrate utilization (g/g).
The organism showed 90% yield efficiency based on substrate consumed.
In these cases, however, substrate selectivity is not a general property and will vary based on substrate levels and substrate-dependent kinetic interaction rates.
The corresponding growth rate is based on carbon substrate concentration, nitrogen and light availability.
Water solution containing 0.63 50 ppm of malachite green can be readily detected by SERS based on substrates of Ag AAO nanoparticle arrays, exhibiting linear-like relationship between SERS intensity and concentrations, Fig. 6b.
These methodologies included those based on the average substrate concentration, those based on the instantaneous substrate concentration, as well as those based on Lagrangian particle tracking.
Based on the interfacial substrate concentration, an analytical rate equation for initial rate of the reaction was derived and confirmed from the experimental data.
Different steady-states of dehalogenation were achieved based on the concentration of substrates added, with effective transformation to ETH obtained when ample electron donor equivalents were provided.
NEU4 was tested with GM3 and 4MU-NeuAc substrates, based on its substrate preference.
Activity was calculated as micromoles per minute product produced (per g of protein in cell extract), based on the concentration of D-sorbitol, xylitor or D-gluconate produced (depending on the substrate(s)).
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