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The cellular components in which most proteins are located are the cellular membranes and the nucleus, a fact additionally present in their categorization based on subcellular location.
Based on subcellular location annotations in Echolocation, EcoCyc and Uniprot (discrepancies and unknowns settled through PSORTb and TMHMM), all pORFs and protein complexes were assigned to one of four compartments: Cytosol, inner membrane, periplasm, and outer membrane [ 9, 37- 40].
In the present study, proteins were first fractionated based on subcellular location, followed by fractionation of peptides based on pI, which indeed greatly enhanced the proteomic coverage in comparison to the previous study [ 25].
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Mean score was subsequently analysed based on subcellular localisation.
It was converted to a layered network based on subcellular localization information.
Here, we delineate possible functional roles based on subcellular localization for SUMO-1 and SUMO-2/3.
Some experiments based on subcellular fractionation, substrate-specific activities, and end-product analyses indicated this location [ 40- 43], while others did not [ 44- 46].
All the network's proteins were categorized based on their subcellular location.
We grouped the proteins based on their subcellular location and clustered them using the MCL algorithm in order to detect functional modules.
But if the peripheral membrane proteins are examined based on their subcellular location the case is different, since 40% of extracellular peripheral membrane proteins have interactions with at least one drug.
All proteins were categorized based on their subcellular location in the following categories: cytoplasm (396), endomembrane system (182), lipid-anchor (25), membrane (239), mitochondrion (143), nucleus (851), peripheral (242), and secreted (228) including (39) extracellular peripheral proteins.
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