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The model system used is based on subcellular fractionation of rat brains followed by ligand binding and functional measurements.
Some experiments based on subcellular fractionation, substrate-specific activities, and end-product analyses indicated this location [ 40- 43], while others did not [ 44- 46].
First, there is evidence based on subcellular fractionation and in vitro studies that 5S rRNA is capable of being imported into mammalian mitochondria [56 59].
Our findings based on subcellular fractionation and immunocytochemical staining indicate that nSMase1 and its phosphorylated form localized primarily to the Golgi apparatus, ER, and nuclear fractions in Jurkat T cells under stress conditions and upon Fas stimulation.
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In the present study, proteins were first fractionated based on subcellular location, followed by fractionation of peptides based on pI, which indeed greatly enhanced the proteomic coverage in comparison to the previous study [ 25].
Mean score was subsequently analysed based on subcellular localisation.
There was overlap between OPTN and GM130, replicating the Golgi localisation of OPTN found on subcellular fractionation and immunoprecipitation.
One interpretation of this discrepancy is that part of Mt STAT3 is lost upon mitochondria isolation based on different subcellular fractionation protocols.
Leptospiral OMP identification has relied on subcellular fractionation methods, including Triton X-114 detergent extraction-phase partitioning and the isolation of OM vesicles [36] [39].
Our study, relying on subcellular fractionation, allows a more precise localization.
Based on import studies, subcellular fractionation, and target sequence prediction, it is suggested that in plants the biosynthesis of aromatic amino acids is predominantly localized in plastids (for review, see [ 29]).
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