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A class of depth-resolved imaging techniques based on Structured Illumination Microscopy (SIM) have been proposed to select a particular imaging plane and to reject out-of-plane background for standard wide-field microscopy [ 23– 25].
Corneal sectioning microscopy was performed on a home-developed microscope, based on structured illumination, which was specifically adapted for fluorescence imaging.
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A home-made sectioned microscope with a fluorescence channel based on structural illumination microscopy was used for imaging the labeled pathogens.
This piece is based on structure, not note by note.
This technique requires the acquisition of two fluorescence images: one with uniform illumination, and one with structured illumination.
A particularly robust and efficient solution for far-field super-resolution that also relies on patterned excitation is structured illumination microscopy (SIM).
We first acquired structured illumination data on fluorescent beads.
For this, we employed structured illumination microscopy (SIM) on live cells.
Superresolution structured illumination microscopy was performed on a Zeiss ELYRA S.1 microscope equipped with a 63× Plan-Apochromat objective (1.4 NA).
Cells were mounted in Prolong Gold, and immunofluorescence was imaged on a Zeiss Axiovert 200 microscope equipped with an Apotome structured illumination system under a 63X/1.4 NA objective.
Single time point live imaging was carried out either on a Zeiss LSM 710 or a Zeiss Imager Z1 with an Apotome2 structured illumination unit.
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