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Cases and controls were carefully adjudicated based on stringent phenotype criteria, and the companion genotyping data underwent substantial QC and filtering.
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B cell culture wells were selected based on stringent criteria (OD > 75% positive control antibody), using the cell-based ELISA.
Segregation of articles to be considered for review was based on stringent inclusion and exclusion criteria.
Test datasets were 1) all new genes based on stringent mapping criteria, 2) Type I New genes using stringent criteria, 3) Type II New genes based on stringent mapping criteria.
In this study, the diagnosis of asthma was based on stringent case definitions.
Firstly, SNP prediction from NGS data should be based on stringent parameters.
A multi-stage cluster sampling technique was used to identify the participants, based on stringent criteria.
ecade ago and were not based on stringent serologic criteria (11– 11).
Overall, there is an urgent need for a common workflow based on stringent criteria, to be implemented by researchers who face the challenge of in-depth analysis of mtDNA sequences, which would allow them to recognize the influence of few variants on the phenotype, thus facilitating the functional assay.
Based on stringent statistical criterion (FDR<0.0005) and/or biological function of interest, we validated selected genes using real-time PCR.
Protein homologs were identified based on stringent cutoff values (E-values < 10-40), and on the score to sequence length ratio according to David et al. (2008) [ 31].
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