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Population sizes were estimated from thawed samples based on standard serial dilution plating procedure.
Absorbance at 450 nm was determined with a plate reader; ferritin concentrations were calculated based on standard curves developed within the same plate and corrected for sample dilution.
ES cells were grown at 37°C in 95% air 5% CO2 on MEF obtained from TgN DR4 1Jae/J mice (http://jaxmice.jax.org/strain/003208.html) at all times except one passage on gelatinized tissue culture plates before aggregation based on standard protocols [38].
A 3-fold dilution series from pooled cDNA were included in each plate for each gene and efficiency (E) of PCR reaction for each set of primers was calculated based on standard curve method according to the formula E = 10 -1/slope -1*100.
Bacteria identification was based on standard methods.
Hit selection was based on "standard scores".
Based on standards from US EPA, OECD and ASTM.
Different finite element plate models based on classical, standard and improved shear deformable plate theories, three-dimensional elasticity equations or their combinations have been developed.
The results were expressed as relative units (RU) based on a standard curve run on each plate using an automated calculation program (MultiCalc; Wallac).
Using the Sector 2400 instrument (MSD) and manufacturer-supplied reagents, the cytokine concentrations were determined based on a standard curve generated for each plate.
Absorbances were read on a SpectraMax 250 plate reader with Softmax Pro (Molecular Devices, Sunnyvale, CA), and the concentrations were expressed as picograms per milliliter based on the standard curve obtained for each plate.
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