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Compounds concentration in tissue samples were determined based on standard curves prepared by serial dilutions (200 0.01 μM) of each of the compound in MeOH ACN (1 1).
The miRNA copy number per cell was estimated based on standard curves of synthetic miRNAs.
Cytokines were quantified based on standard curves obtained using an ELISA reader (Dynex, Chantilly, VA).
Absorbance at 450 nm was determined with a plate reader; ferritin concentrations were calculated based on standard curves developed within the same plate and corrected for sample dilution.
All experiments were performed in a blinded manner and in duplicates, and the HER2 concentration was calculated using their average optical densities based on standard curves.
PCR efficiency of each primer set was determined based on standard curves of serial 10fold dilutions of cDNA from Raji or U937 cells.
Similar(22)
PG I and II levels were calculated based on standard curve.
The levels of MDA was calculated based on standard curve constructed with 1,13,3- tetra ethoxy propane.
Standard curve was established with pure gemcitabine and release of drug was determined based on standard curve.
Sample values were normalized based on standard curve for each protein and data were calculated using Luminex software.
The absolute numbers of MDV genome per 100 ng of spleen DNA were calculated based on standard curve.
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based on standard data
based on standard patterns
based on standard protocols
based on standard algorithms
based on standard radiographs
based on standard methods
based on standard tests
based on standard procedures
based on standard criteria
based on standard scores
based on standard rules
based on hypotrochoid curves
based on standard techniques
based on standard definitions
based on principal curves
based on standard rates
based on standard metrics
based on standard patents
based on standard recommendations
based on standard guidelines
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