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Nevertheless, one third of positive predictions based on standard alignments were not detected by the other variant.
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For both datasets, proper alignment is applied to the images based on standard face landmarks.
Hit selection was based on "standard scores".
Bacteria identification was based on standard methods.
We employed two separate tests for primer-primer interaction, one based on a standard local alignment to detect stretches of complementary sequence, the other based on the worst-case ΔG of the 3'-tail of one primer interacting somewhere along the strand of another primer.
A recent re-implementation of M-Coffee in the SeqAn (Doering et al., 2008) alignment library shows that the multiple alignment step of M-Coffee is about twice faster than standard consistency-based aligners based on pair-wise alignments like ProbCons or Promals (Rausch et al., 2008).
We then constructed multiple alignments of peptide sequences using ClustalW and constructed nucleotide alignments based on the peptide alignments.
Since sequence alignments based on structural alignments have been long considered to be the gold standard, it is reasonable to expect that substitution matrices based on structural alignments can lead to better performance in detecting remote homologs.
The SILVA seed alignments are based on alignments published by the ARB project in 2004.
Transposition of ligands is based on accurate alignments of three-dimensional amino-acid patterns or of their corresponding functional groups in the proteins' binding sites; due to their local nature in the binding sites, such alignments may not be possible with standard sequence or structure alignment approaches.
Two families were identified based on LTR alignments.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com