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The purity of monocytes was greater than 90% based on staining with anti-CD14 antibody.
The purity of mouse NK cells were greater than 90% based on staining with NK1.1 and DX5 antibodies.
A common method for detection of apoptotic cells is based on staining with Annexin-V and a DNA-binding dye that does not penetrate intact cell membrane (e.g. PI).
White outlines of the EAD shown in figures were drawn based on staining with DAPI.
For all enzymes, purity was determined to be >95% by SDS-PAGE gel electrophoresis based on staining with Coomassie Blue.
Based on staining with MTT, the percentage of viable pollen grains in just dehisced anthers was 50 ± 4 % for B. crispa, and 81 ± 2 % for B. officinalis.
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The effect of SAG or PAC on the proliferation of lung cancer was assessed using a cell proliferation assay based on staining cells with crystal violet as described before [20].
ALDHhigh positivity was selected based on staining parallel cells with DEAB inhibitor of ALDH activity (Additional file 2: Figure S2).
In this study, we aimed to establish a methodology based on staining dead cells with the fluorescent markers Sytox and propidium iodide to evaluate the viability of C. elegans and its further use on testing of novel anthelmintic compounds for control of human and animal nematode infections.
TP53 immunoreactivity was dichotomised into positive and negative, based on staining of malignant nuclei, with a threshold of 10% [ 26].
p53 immunoreactivity was dichotomised into positive or negative based on staining of malignant nuclei, with a threshold of 10%.
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