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The methods originating from environmental microbiology are based on staining and counting of cells for the determination of total cell biomass (acridine orange and DAPI), physiological state of cells (LIVE/DEAD® BacLight™) and activity of cells (reduction of the redox dye CTC and microautoradiography).
Pyramidal neurons were selected based on staining and morphology.
Cells were counter stained with DAPI (Invitrogen) and scored based on staining and morphology.
Vessel layers were selected based on staining and morphology.
Vascular smooth muscle cell layers were selected based on staining and morphology.
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Two study pathologists (HH and MM) blinded to case status reviewed the stained slides and scored each case based on staining intensity and percent of cells on slide showing evidence of overexpression.
With regard to the evidence based on staining, radiolabeling and ultrastructure, one alternative scenario is that the loriciferan tissues within their lorica (exoskeleton) were actually dead, degraded to varying degrees and inhabited by living anaerobic bacteria, archaea, and/or fungi.
Two pathologists blinded to clinical information evaluated and scored PRUNE2 staining based on staining extent and intensity [6].
Similar to previous studies (Destro et al, 2006; Zucali et al, 2011a, 2011b), the results were interpreted using a system based on staining intensity and on the number of stained cells.
Expressions of BRCA1, RRM1, RRM2, p53R2, and TUBB3 were evaluated semiquantitatively based on staining intensity and proportion.
Nuclear immunoreactivity for PPAR γ was based on staining intensity and graded as 0, no staining; 1, mild-to-moderate staining; and 2, strong staining.
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