Sentence examples for based on stable reference from inspiring English sources
More importantly, proper normalization of RT-qPCR data based on stable reference genes is critical for miRNA quantitation, because variations can stem from many sources, such as differences in sample procurement, stabilization, RNA extraction, and target quantification.
To control for differences between sample input, each lane was then normalized to a reference gene (SMYD3 was chosen based on stable expression between glioblastoma and control samples).
They provide the building blocks of information systems: syntactic interoperability and semantic interpretability [ 30] In this two-layered approach, a repository based on a stable reference model, the first layer (see paragraph below), contains just generic knowledge and business rules [ 31].
As calculated based on the reference gene EF-2, an internal control whose expression was stable under all conditions tested, MCL1 expression increased approximately 4-fold in cells 8 h after S. aureus phagocytosis (Fig. 8A).
P0 was selected as the reference gene based on its stable expression across the circadian cycle relative to four other commonly used reference genes (βactin, 18S, cyclophilin, gapdh).
Expression levels for all candidate genes were computed based on the stable expression level of the reference gene.
Data were analysed based on the stable expression level of the reference gene according to method of Livak and Schmittgen.
Expression levels for all candidate genes were computed based on the stable expression level of the reference gene according to Pfaffl method [ 88].
Normalization of the target genes was based on the stable expression of the following reference genes: ATP Binding protein (EgrABP, Eucgr.I01239) and Zinc ion Binding Protein (EgrZIB, Eucgr.D02582).
