Extraction efficiencies from SPE and POCIS extracts were 40-100%, but after correction with internal standards, based on spike-and-recovery experiments, efficiencies were 90-11090-110% not shown).
Sorted units were manually confirmed and classified as single units (SU), multi-units, or artifacts based on spike shape and variance, peak-amplitude-to-noise level, the inter-spike interval (ISI) distribution of each cluster and presence of a refractory period.
After spike sorting as previously described (Takahashi et al., 2003; Takahashi and Sakurai, 2009; Takahashi, 2013), putative principal cells were distinguished from putative fast-spiking cells based on spike width (0.4 ms) and average firing rate (5 Hz).
Another approach to validate recovery and accuracy is based on spike preparations of analyte and vehicle in the diluent.
Principal excitatory units (putative pyramidal cells) and inhibitory units (putative interneurons) were discriminated based on spike duration, firing rate, and autocorrelogram [37], [42] [44] [45](see Materials and Methods for details).
Based on spike waveforms, firing rates, and inter-spike intervals, the recorded CA1 units were divided into two classes: principal excitatory units (putative pyramidal neurons) (Figure 3) and inhibitory units (putative interneurons) (Figure 4).
"This paper presents a computer model of cortical broadcast and competition based on spiking neurons and inspired by the hypothesis of a global neuronal workspace underlying conscious information processing in the human brain.
The text now reads: "Single units were classified from multi-unit activity based on spike shape, separation of principal component projections, and the presence or absence of a refractory period between successive spikes".
DOI: http://dx.doi.org/10.7554/eLife.02743.006 Single units were distinguished from multi-unit activity based on spike shape, separation of principal component projections, and the presence or absence of a refractory period between successive spikes.
This population was alternatively cultivated within a mixture composed of at least three distinct varieties and as a pure variety after a selection step based on spike type.
Statistical analysis was done, using the software R [ 74] involving data normalization based on spike-ins and detection of differential gene expression.
