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PDCD4 is one of the phosphoproteins that appeared to be induced by CXCL12 stimulation based on spectral counts (Figure 5A and Table 2).
More recently, we implemented Significance Analysis of INTeractome (SAINT) within ProHits to provide scoring of interactions based on spectral counts.
For each site, phosphorylation levels were based on spectral counts [38].
dNSAFs were calculated based on distributed spectral counts, in which shared spectral counts were distributed based on spectral counts unique to each isoform.
To increase the stringency of our analysis, the Rab proteins were semi-quantitatively profiled based on spectral counts [41] over the entire Nycodenz density gradient separating the post-nuclear fraction of a rat liver homogenate.
Looking for negative correlations between miRNA level and protein expression based on spectral counts, we found that a higher number of miRNAs, 114 (97.4%) had a direct target protein identified (Table S2).
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Comparative analysis based on spectral counting was accomplished using Scaffold (Proteome Software, Inc., Portland, OR).
TIMP1 occurred predominantly in the secreted compartment of all cell lines, consistent with predictions based on spectral counting.
Based on spectral count, the bait protein was usually the most highly identified protein in each control-subtracted sample, evidence for the efficacy of the pulldown.
Statistical analysis was based on spectral count [52], however the normalization procedure was specific to in-gel and in-liquid digested samples.
In our study we also observed a good correlation between estimates of abundance of tissue inhibitor of metalloproteinase 1 (TIMP1) and epithelial cadherin (CDH1) based on spectral counting and assessment based on Western blot analysis.
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based on play counts
based on average counts
based on spectral filters
based on spectral invariants
based on actual counts
based on fibre counts
based on raw counts
based on spectral features
based on spectral transforms
based on spectral data
based on spectral characteristics
based on total counts
based on spectral methods
based on high counts
based on periodic counts
based on simple counts
based on frequency counts
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