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Comparative analysis based on spectral counting was accomplished using Scaffold (Proteome Software, Inc., Portland, OR).
TIMP1 occurred predominantly in the secreted compartment of all cell lines, consistent with predictions based on spectral counting.
In our study we also observed a good correlation between estimates of abundance of tissue inhibitor of metalloproteinase 1 (TIMP1) and epithelial cadherin (CDH1) based on spectral counting and assessment based on Western blot analysis.
With this caveat in mind we employed the two semi-quantitative methods based on spectral counting and peak intensities to identify phosphopeptides that are apparently altered in the 2- and 50-cGy samples relative to the sham irradiated control (Figure 1B).
We next performed a relative quantification of the NKX3.1 interactome based on spectral counting.
NSAF (normalized spectral abundance factor), a metric based on spectral counting [ 86], was used to quantify protein expression.
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PDCD4 is one of the phosphoproteins that appeared to be induced by CXCL12 stimulation based on spectral counts (Figure 5A and Table 2).
More recently, we implemented Significance Analysis of INTeractome (SAINT) within ProHits to provide scoring of interactions based on spectral counts.
For each site, phosphorylation levels were based on spectral counts [38].
dNSAFs were calculated based on distributed spectral counts, in which shared spectral counts were distributed based on spectral counts unique to each isoform.
Based on spectral count, the bait protein was usually the most highly identified protein in each control-subtracted sample, evidence for the efficacy of the pulldown.
More suggestions(17)
based on spectral resolution
based on double counting
based on spectral subtraction
based on spectral partitioning
based on spectral classification
based on spectral clustering
based on spectral reflectance
based on direct counting
based on spectral substraction
based on spectral decomposition
based on spectral filtering
based on spectral envelope
based on spectral emissivity
based on manual counting
based on spectral estimation
based on spectral representation
based on spectral characteristic
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