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Earlier we used data based on sonicated mitochondrial samples[ 21].
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These three mutant proteins retained the proper mitochondrial localization based on immunohistochemistry and mitochondrial subfractionation studies.
Based on the mitochondrial DNA of the women descended from those in the first migration, Dr. Wallace estimated it occurred 20,000 to 30,000 years ago.
But the family tree based on human mitochondrial DNA does not trace back to the thousand women in this ancestral population.
Standard approaches are based on sequencing mitochondrial and nuclear targets.
Therefore, clustering based on the mitochondrial gene marker represents the population structure of effective populations.
Their phylogenetic analysis is based on two mitochondrial genes, nad2 and mutS.
Phylogenetic analyses of 74 individual wasps, based on six mitochondrial and nuclear genes, revealed three lineages.
Our newly estimated divergence times based on the mitochondrial genomes are shown in Fig. 3.
This is in contrast to a previous phylogeny based on the mitochondrial 16S rRNA gene [ 111].
The latter effect was confirmed based on uncoupled mitochondrial respiration rate measurements (data not shown).
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