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This method, which is based on size separation in neutral gels and sequence separation in denaturing gradient gels followed by hybridisation analysis with mini- and microsatellite core probes, allows the simultaneous analysis of hundreds of allelic fragments in a very short time.
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Local dispersity can also play a role in other separation techniques where separation is based on size, such as field-flow fractionation or hydrodynamic chromatography.
Furthermore, the adsorption of a single protein from mixtures based on size- and shape-selective separation can open up new ways to produce micro-objects that suit a given protein encapsulation design.
There are two basic types of membrane separations: one involves the mechanical separation of sample components based on size exclusion.
Consequently, the trade-off relationship between permeability and selectivity for conventional ultrafiltration membranes where separation is based on size exclusion solely could be overcome.
Most of them include a separation step based on size (density gradient or filters) or biological characteristics (expression of epithelial- or cancer-specific markers), followed by the detection using ICC or molecular assays [ 36].
Filtration enables separation of mixtures based on size differences between the mixture components passing through a porous structure (filter/membrane) under the influence of an external force field, typically pressure.
The separation is thus based on size exclusion, which is why IUPAC recommends using the term SEC instead of GPC.
This study provides an easy and efficient method of separation of biogenic nanoparticles based on size and shape effects.
Separation of proteins, including proteoglycans, is largely based on size.
Following the enzymatic digestion, an SEC separation was performed to determine the size dispersion of the resulting heparin oligosaccharides and fractionate the products based on size.
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