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In this article, we extracted peptidome fractions (molecular weight <5,000 Da) from 92 individuals using the well-established and reproducible one-step peptidome enrichment method based on size exclusion chromatography (SEC) [22], [23] and provided them to the label-free mass spectrometric quantification analysis combined with the statistical analyses on Expressionist proteome server platform.
There are two basic types of membrane separations: one involves the mechanical separation of sample components based on size exclusion.
Selectivity enhancement mechanism was proposed, based on size exclusion principle.
Here, we presented a lab-on-a-chip filter device to isolate plasma and viruses from unprocessed whole blood based on size exclusion without using a micropump.
A second purification step, based on size exclusion chromatography, was then utilized, being effective in the elimination of other previously identified contaminating proteins.
Consequently, the trade-off relationship between permeability and selectivity for conventional ultrafiltration membranes where separation is based on size exclusion solely could be overcome.
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A microfluidic device to separate bacteria from blood cells based on size-exclusion through cross-flow channels was designed and performance tested using fluorescently-labeled, heat-killed Escherichia coli spiked into whole blood.
Based on size-exclusion filtration we determined that the extracellular AGEs are smaller than 3 kD.
The determination of the oligomeric state of proteins is normally based on size-exclusion chromatography, ultracentrifugation, X-ray scattering or dynamic light scattering.
The hydrogenase was obtained as a homogeneous protein of MW ∼150,000 Da (based on size-exclusion chromatography, data not shown), also similar to that of the native enzyme, and contained the four different protein subunits (Fig. 6a) [11].
The mechanism of ion rejection by these modified membranes was based on size-based exclusion rather than charge-based separation.
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