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Then the ligated products were selected based on size by running 10% polyacrylamide gel.
In this method, the frequency of the sinusoid wave is held constant, and particles can be selected based on size by changing the frequency of the square wave using a simple electrode design [ 106].
The GeXPS system was used to separate PCR products based on size by capillary gel electrophoresis and to measure their dye signal strength in arbitrary units (A.U). of optical fluorescence, defined as the fluorescent signal minus background.
The GeXP system was used to separate PCR products based on size by capillary gel electrophoresis and to measure their dye signal strength in arbitrary units of optical fluorescence, defined as the fluorescent signal minus background.
The ligated products were selected based on size by running 10% polyacrylamide gel and purified for the final PCR amplification (94°C for 30 s, 60°C for 20 s, and 72°C for 20 s) for 20 cycles.
The GeXPS machine (Beckman Coulter, Brea, CA, USA) was used to separate PCR products based on size by capillary gel electrophoresis and to measure their dye signal strength in arbitrary units (AU) of optical fluorescence, which is the fluorescent signal minus background.
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Peak fractions containing Fur protein from the ion-exchange procedure were collected and further purified based on size exclusion by using a Sephacryl-200 column (buffer C [50 mM sodium phosphate, 200 mM NaCl, pH 8.0]).
Only 2 μl of the cy5-labeled PCR amplified fragments were separated by capillary electrophoresis based on size and detected by the GeXP system.
This has been corroborated, based on size and weight, by jewelers familiar with the material Apple is using to make its Apple Watch Edition pieces.
An intuitive approach for separating cells based on size exclusion is by filtration.
In termites, partner selection based on size is supported by findings of Shellman-Reeve (1999), who observed that females of Z. nevadensis choose males with big heads, whereas males choose females according to body mass and lipid mass.
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