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RNA was extracted from 72 BNYVV isolates from Asia, Europe and North America, and the type of each isolate determined using an established detection method based on single strand conformation polymorphisms (SSCPs).
This unique feature distinguishes PECVD from TCVD and opens up the possibility of making nanodevices based on single strand CNT.
Based on single strand conformation of polymorphism analysis after RE (AluI and RsaI) digestions of 16S rDNA, two different evolutionary schemes for the two Bacillus species, B. cereus and B. licheniformis were proposed [60].
Primers for the SNPs identified in the Maq-default analysis were designed to amplify ~150-200 bp fragments as the initial screens were based on single strand conformation polymorphism (SSCP) of PCR products.
In the study of Pawlotsky et al. [ 35] based on single strand conformational polymorphism and in the study of Saludes et al. [ 34] based on clonal sequencing, no significant differences in pretreatment HVR1 complexity were observed between responders and non-responders.
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The mononucleotide GC content of sequences was determined from a count of each nucleotide type in the sequence, based on single stranded DNA.
Also by replacing the conventional gel-purification protocol with one based on single-strand DNA separation, higher purity of ligated versus non-ligated DNA can be achieved, resulting in a more sensitive assay (data not shown).
This model is based on single-strand assimilation models of Leung et al. [96], and Ellis et al. [97]; Court et al. [98], for yeast and phage λ Red-mediated recombination, respectively.
Examined bases were called wild-type based on single-strand evidence or were left as no-calls depending upon the feature intensity profile.
The evidence against heterokaryosis brought forward by Stukenbrock and Rosendahl (2005a) is based on single-strand conformation polymorphism and does not offer the resolution to distinguish between nucleotide differences within variants.
In an earlier Health Canada risk assessment on OTA by Kuiper-Goodman and Scott (1989), it was concluded that the available data supported that OTA was not mutagenic, but that it was weakly genotoxic to mammalian cells based on single-strand breaks.
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