Sentence examples for based on single sequences from inspiring English sources

For the same reason we also decided to apply conventional probes for the detection of pandemic A(H1N1) viruses, because in April 2009 the assay designs had to be based on single sequences without any knowledge concerning the viruses sequence variability.

Existing methods for the prediction of interactions are all based on single sequences.

We have also shown both for a vertebrate snoRNA and for four bacterial sRNAs that the addition of evolutionary information from multiple sequence alignments improves the performance in comparison to methods based on single sequences.

Here, we examine if it is possible to use the consensus of topology prediction methods that are based on single sequences to obtain a similar accuracy as the more accurate multiple sequence-based methods.

However, applications of such tools have some limitations, as they are based on single sequence secondary structure and energy based features, whose accuracy and reliability drop drastically with increase in the length of sequences [ 21, 22].

Despite their wide-ranging utility, ESTs have two inherent drawbacks: (1) they are based on single sequence reads making them vulnerable to sequencing errors, and (2) they are generated from cDNA libraries that may contain unexpressed or incompletely spliced sequences derived from heteronuclear RNA or other artifacts.

Thus, the best discriminatory results based on single gene sequences were those obtained from β-tubulin and calmodulin phylogenetic analysis which placed 26 taxa within subgenus Circumdati section Nigri, further dividing them into 5 main clades, grouping all uniseriate species (7) in only one clade (the A. aculeatus clade) [ 13, 45].

Therefore, new sequencing technologies based on single molecule sequencing might offer further opportunities to eventually obtain a genome build and SNP chip that in actuality covers the vast majority of the chicken genome.

According to an estimate based on single sample sequencing, it would require coverage of at least 20X to detect 99%% of the heterozygous variants [ 55].

The primers were designed on the basis of IGS sequence (Intergenic Spacer of rDNA), a multi-copy region in the genome that permits to enhance the sensitivity of the assay in comparison with PCR assays based on single-copy sequences.

The specific primers were designed on the basis of IGS sequences (Intergenic Spacer of rDNA), a multicopy region in the genome that permits to enhance the sensitivity of the assay in comparison with PCR assays based on single-copy sequences.