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Localized factors relating to differential limb growth may play a larger role in the distribution and density of osteocyte lacunae than previously thought, and are potentially biasing the interpretations of analyses based on single samplings of isolated specimens.
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All values are based on single analyses.
Since our analysis was based on a single sampling event using all precautions to avoid possible contamination we can potentially link the presence of residual DNA to carry over through the organisms present in the larval community we sampled.
Based on fast controllers predesigned in this paper for the most common situations (50/60 Hz grid frequency, single and three-phase systems, presence of odd and/or even harmonics) the new proportional integral controller parameters are obtained using analytical formulas for new operation conditions (input amplitude and sampling frequency).
Alternative selection criteria and phenotyping methods would have to be developed for progressing from a fleece-oriented approach based on a single universal sampling point towards methods embracing 2D variability.
Concentrations were calculated based on sample mass, sampling duration, sampling rate and recovery efficiency.
Estimates of contemporary effective size (roughly, Ne that applies to the time period encompassed by the sampling effort) can be based on either a single sample (Hill 1981; Pudovkin et al. 1996) or two samples (Krimbas and Tsakas 1971; Nei and Tajima 1981).
Third, the short read libraries used here obviously will capture only a restricted part of the total transcriptome as they are based on a single tissue and only two sampling time points.
Estimates based on this sampling approach may differ from sampling approaches designed to provide population estimates.
The sample size was calculated based on the sampling method recommended by WHO for drug resistance surveys in TB [ 10].
All samples were coded as valid, flagged, or invalid, based on the sampling period and field technician comments.
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