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Therefore, constructing a "skeleton bin map", which combines the co-segregating markers into one bin and separates adjacent bins based on single recombination events, is an effective approach for capturing all of the recombination events using saturated markers [ 39], which increases the power, accuracy and resolution needed to identify QTLs [ 40– 40].
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Mutations based on single cross-over recombination are inherently unstable, whereas insertion of the group II intron in the anti-sense orientation, relative to the gene, results in permanent inactivation (Heap et al. 2007).
Thus, this duplication cannot be reliably predicted based on single SNPs or multi-SNP haplotypes due to simple recombination, genotyping problems due to heterozygous genotypes, and reversion to the non-duplicated state via non-allelic homologous recombination.
All values are based on single analyses.
Thus, our results warrant caution in classifying EBV strains: recombination events may confound relatively simple classifications based on single-gene polymorphisms such as those found in the EBNA-1 gene.
This model is based on single-strand assimilation models of Leung et al. [96], and Ellis et al. [97]; Court et al. [98], for yeast and phage λ Red-mediated recombination, respectively.
The telomere length dynamics of telomerase-negative immortalized cells suggested that this mechanism is based on recombination: single telomeres undergo dramatic length fluctuations during cycles of cell division, with rapid shortening and rapid lengthening events (3, 4).
The traditional gene disruption strategy based on homologous recombination usually takes two steps: one single crossover followed by a second crossover.
Recombineering is a DNA engineering methodology based on homologous recombination in Escherichia coli.
Gene targeting (GT) based on homologous recombination (HR) enables the modification of desired genomic DNA sequences.
We have previously developed a chromosome-splitting technique based on homologous recombination inSaccharomyces cerevisiae.
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