Sentence examples for based on single peptide from inspiring English sources
Because the potential for erroneous identification based on single peptide identification is greatly increased, proteins not present in the plasma proteome selected in this study were removed from the 292 proteins.
A total of 292 proteins in plasma were identified by Jin Young Kim et al using both SEQUEST searching[24] and a protein data filtration method based on correlation (MWcorr) between the experimental (1-DE) and the theoretical MW[17], although more than half of the assignments were based on single peptide identification.
Interestingly, it appears that automatic elimination of all protein identifications based on single peptide hits results in the disproportionate depletion of positive identifications in target and decoy databases, which has driven a recent argument that protein identifications should be subject to estimated false-positive rates, similar to the current standard commonly employed for peptides [44].
Of these 569 proteins, identification of 367 proteins was supported by 2 or more peptides, while 202 protein identifications were based on single peptide evidence.
Of these, 28 differentially expressed proteins were identified by multiple peptides and 17 protein identifications were based on single peptide evidence with multiple PSMs.
The number was much smaller than that of proteins identified by the same method with tissue extract proteome [ 15], which was accounted for by the small number of the proteins identified based on single peptide match.
In contrast, the SmD1 ELISA and the SmD3 ELISA are based on single peptides derived from the SmD sequence [ 14, 15].
This FDR is better than recently reported rates [ 25] and although 4,567 (58%) of the protein identifications in this study were based on single-peptide matches, the low FDR provides a high degree of confidence in these identifications.
As a result, quantifications of the majority of kinases were based on single unique peptides.
Protein hits based on a single peptide identification with Mascot score higher than the significance level (>15) were retained after manual validation.
