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Multilocus haplotypes may capture the LD information in a gene better than methods based on single loci [3].
The combined phylogenetic analyses of multiple genes have become popular due to the poor resolution of phylogenies based on single loci [30], and have successfully inferred better-resolved phylogenies within the major taxonomic groups, including animals [31], plants [32], fungi [33] and bacteria [34].
However, the standard DNA barcoding protocols used to infer putative species are based on single loci and do not test species limits with models tracking speciation and population genetic processes e.g., [ 20].
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The original analysis based on single locus models revealed only three genes associated with T2D: PPARG, KCNJ11 and TCF7L2 [ 1].
We have previously shown that P. acnes comprises a number of distinct evolutionary lineages, designated types IA, IB, II, and III, based on single locus phylotyping [ 17, 18].
Many previous estimates of the staphylococcal phylogeny have been based on single locus gene trees, which in many cases exhibit differing topologies.
The MLVA profile of the isolates was also subjected to a minimum spanning tree (MST) analysis in BioNumerics (MLVA plugin 2.1), illustrating the relationship and possible mutation pathways within the clusters based on single locus variations (SLV).
When more than one organ from the same pig was positive for M. avium subsp. hominissuis, the MLVA profile of the isolates was subjected to minimum spanning tree (MST) analysis in BioNumerics 6.1, illustrating the relationship and possible mutation pathways within the clusters based on single locus variations (SLV).
These results demonstrate that parameter estimates based on single-locus population genetic data have large deviations regardless of sequence length or true value of the parameter of interest.
Doubts have also arisen concerning species identification and delimitation based on single-locus DNA sequences, which frequently result in problematic under- or overestimation of species [ 16- 18].
We also corroborate the sister relationship of Calamonastes and Camaroptera, which had previously been inferred based on single-locus analyses only [ 37, 38].
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