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To facilitate high-throughput characterization of genome structure based on shotgun sequence reads, we have developed an automated sequence read classification pipeline (SRCP).
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We propose that the most effective SNP discovery strategy for the rat would be one based on shotgun sequencing of a single wild-derived sample and subsequent low-cost high-throughput genotyping of the resulting candidates in the laboratory strains of interest.
In these experiments, the composition of the gut microbiota was monitored over a one-year period using a culture-independent method based on shotgun sequencing of 16S rRNA clone libraries.
Our analysis is based on shotgun sequencing data of a HeLa cell line at moderate depth.
The published silkworm genome sequence represents one of the first attempts to sequence and assemble a lepidopteran genome mainly based on shotgun sequencing read data.
We found that the microbiota composition of 16S rRNA gene sequences obtained using Sanger, 454 and SOLiD sequencing provide results comparable to the result based on shotgun sequencing.
The microbiota composition of 16S rRNA gene sequences obtained using Sanger, 454 and SOLiD sequencing provided results comparable to the result based on shotgun sequencing.
One of the individuals in this family group gave excellent results in a recent study based on shotgun sequencing and WGS [ 34].
In this study, we compare different computational methods for improving the accuracy and sensitivity of aDNA sequence identification, based on shotgun sequencing reads recovered from Pleistocene horse extracts using Illumina GAIIx and Helicos Heliscope platforms.
Genome sequencing of swine goes back to the early 2000's, when the Sino-Danish Pig Genome Project was initiated and subsequently a 0.66× coverage genome survey, based on shotgun sequencing, was published [ 15].
On the other hand, metagenomic approaches are based on shotgun sequencing of DNA from species mixtures, which are used mainly in prokaryotic research, with some recent examples of unicellular eukaryotes (see Raven 2012).
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