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Standard curves based on serial dilutions of samples were established to correct for differences in efficiencies between primers.
The LightCycler 3 Run software (Version 4.24) generated a gene specific standard curve based on serial dilutions of the genomic DNA and copy numbers in all samples representing each time point were quantified.
Target and control primer efficiencies were estimated based on serial dilutions of cDNA template.
For each gene, a calibration curve was generated based on serial dilutions (101 to 102 pg/μL) of plasmid templates.
The generation of a standard curve, based on serial dilutions of fluorometrically quantified PCR products has been shown to be very reliable [ 29].
Based on serial dilutions of quantified PCR products, the detection limit was estimated to be 1.6 gene copies per microliter extracted DNA per PCR.
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To quantify organ-specific TA for the corresponding poplar MPK and MKK genes, standard curves were produced based on serial dilution of each of the pCR2.1 plasmids containing the cloned 3'UTR of each gene, and used to determine the number of transcript molecules as described in [ 69].
We then generate percent inhibition metrics and produce dose response curves based on serial drug dilutions.
A hemagglutination-inhibition (HA-I) assay (Carroll et al. 2007) based on serial twofold dilutions of sera reveals the presence of this natural antibody.
A test based on the serial dilution of depurated and tainted water was undertaken, with barramundi placed within each treatment and subsequently evaluated for their sensory characteristics.
The concentration of inoculated SQR-21 was 1 × 106 cfu/mL based on the serial dilution plate counting method.
More suggestions(15)
based on serial algorithms
based on serial numbers
based on appropriate dilutions
based on serial radiographs
based on serial examinations
based on different dilutions
based on serial changes
based on serial reconstructions
based on serial killers
based on serial measurements
based on serial ECGs
based on high dilutions
based on serial arrays
based on serial rounds
based on serial observations
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