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Importantly, MgC-GEP is based on sequencing technique and can be freely applied in most laboratories without the acquisition of specific equipments.
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Current methods for fish species identification are mainly based on sequencing, a technique that is time and resources consuming boosting the need for the development of more expedite methods.
Z-2 reservoir was interpreted as early transgressive systems tract normal regressive sediments based on sequence stratigraphic techniques used in the modelling.
The Signal-BLAST algorithm performs signal peptide prediction based on sequence alignment techniques.
Despite these recent developments, the use of genome-wide expression techniques based on sequencing (RNA-Seq) in conifer species has remained limited [ 19– 219.
Molecular techniques based on sequencing of the gyrA, gyrB, and rpoB genes (5 ) enabled more precise identification and indicated that all isolates belonged to the Acinetobacter genomospecies (DNA group) 15TU, which is known to be phylogenetically related to A. lwoffii and which has been reportedly isolated from sewage, freshwater aquaculture habitats, trout intestines, and frozen shrimp (6 ).
The RNA-Seq technique is based on sequencing the poly-A RNA fraction and allows for characterizing isoforms from known genes or discovering novel, predicted coding genes [ 16].
Further transcriptome analysis based on novel sequencing technique will enable a more complete survey of the diversity or similarity between apical and basal cells at the transcriptional level and a more accurate answer to this question.
Results show that the technique based on sequence similarity and protein family classification maps transcription factors most efficiently from S. cerevisiae to A. thaliana; therefore, it is the preferred method for general transcription factor mapping.
We attempted to overcome the OG identification problem by combining clustering techniques based on sequence similarity (BLAST algorithm) with phylogenetic methods using a semiautomated pipeline (written in AWK and Perl).
It could thereby substantially improve current TFBS prediction techniques, which are based on sequence conservation and tend to yield too many false-positive hits.
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