Mostly based on sequencing approaches, these other studies reported patterns at the "species" level, whereas our method of investigation was based on fingerprinting, which provides a fuzzy, yet consistent, picture of local microbial communities.
Further studies based on sequencing approaches are needed for clarification.
Our results, based on a Sanger sequencing study of a candidate gene and preliminary data from the 1000 Genomes Project for COMT, show that imputation of untyped markers based on sequencing a portion of the study participants is a reasonable, cost-saving approach for disease mapping and refinement of putative regions detected with GWAS.
The study was based on sequencing total DNA using either the 100 or 150-bp paired-end chemistry and Illumina HiSeq 2000, assembled using the polar bear genome sequence as a reference (Li et al. 2011).
Based on sequence-structure alignment studies, we have folded the amino acid sequence of HuPON1 onto the sixfold β-propeller structure of squid diisopropylfluorophosphatase (DFPase).
Many studies based on sequence-based methods and network-based methods have been made in protein's gene phenotypes identification [ 21– 23].
Because rs3117222 maps 6 kb downstream of the HLA-DPB1 gene, we also tested the independence of rs3117222 with HLA-DPB1 alleles including DPB1*0301, an allele inversely associated with FL based on HLA sequencing studies of the SF-NHL2 study population [ 4].
Another pattern repeatedly recovered in studies based on sequence data [ 8– 12] 12] and a study combining molecular and morphological data [ 29], has been that Papilionidae is the sister of the other papilionoid families, including Hedylidae and Hesperiidae.
The reduced-taxa data set was largely based on sequences used in other studies [ 31, 56], complemented by additional sequences for crucial species (see Additional file 1, Table S1).
Studies based on sequence data do not suffer from SNP ascertainment bias as do studies that are performed using commercially available SNP assays.
