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For reads produced by 454 or Ion Torrent sequencers, low-quality bases were filtered based on sequencing quality scores.
Alignments are filtered based on sequencing quality, mapping quality and mismatch rates.
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The program uses output from pairwise genomic comparisons in MUMmer [40], grouping SNPs and indels, and allowing filtering based on sequence quality metrics (phd_cutoff = 30, phd_avgcut = 30, region_all_threshhold = 20) and size of syntenic region, including flanking length (30 bp), and length to mismatch (1 bp).
Although the correction of sequence data are generally inappropriate for metagenomic analysis, PRICE did implement a feature that filters input data based on sequence quality scores in a user-controllable and dynamic manner.
We define a distance metric between samples based on seven sequencing quality control metrics from Picard (http://broadinstitute.github.io/picard).github.io/picard
Our CNV calling pipeline (discussed further in the Supplementary Material) works as follows: We define a distance metric between samples based on seven sequencing quality control metrics from Picard (http://broadinstitute.github.io/picard ).github.io/picard
The automated data-cleaning function based on sequence read quality (Phred) scores was applied to a public whole human genome sequencing data and we proved the overall mapping quality was improved.
For each Hirudo transcript, for its complement of aligned proteins, each word from each description was assigned a weight based on sequence alignment quality.
As noted above, the likelihood that a homozygous SNP in 4 of 17 murine strains represents sequence error is minimal (<1 in 10 based on sequencing depth and high Phred quality) and manual SNP assessment indicates authenticity of the homosites identified here.
Variants were filtered based on sequence coverage and quality scores.
The resulting ESTs were filtered based on sequence trace file quality, screened for mitochondrial contamination and assembled into contigs using SeqMan II.
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