Suggestions(2)
Exact(3)
Amplification was carried out using primers cagRV17-F 5'-acc ctg gtc ggt aat gga tta tct-3' and cag22-R 5'-tta aga ttt ttg gaa acc acc ttt t-3', which were designed based on sequences retrieved from Genbank using ALIG (scientific and educational software) and ClustalW (BCM Search Launcher, multiple sequence alignments).
Primers were designed using the Oligo Primer Analysis Software [ 27], based on sequences retrieved from the GenBank/EMBL databases.
Based on sequences retrieved from the DeepSeaVent database, primers were designed to confirm, in RT-PCR experiments, the physical counterpart of B. azoricus genes putatively associated with immunity and inflammatory reactions.
Similar(56)
Based on the sequences retrieved from these areas, marine sediments could be considered as a valuable source of bacterial WS/DGAT enzymes with biotechnological relevance.
Primers were designed using Primer Express 2.0 (Applied Biosystems), based on target sequences retrieved from the Affymetrix Probe Sequence Database (Liu et al., 2002).
We searched for all sequences differing from 0 to 8 nucleotides from the preliminary consensus sequence (based on the sequences retrieved from the BLAST search) at the other positions using the server Target Explorer [ 27, 28].
Primers were designed using the Oligo7 Primer Analysis Software (Molecular Biology Insights, Cascade, CO, USA), based on cDNA sequences retrieved from RNA-seq and are listed in Additional file 16: Table S9.
To fully explore their potential as mammalian genome manipulation tools for gene therapy and gene discovery, reliable data sets of target sequence preferences based on targeting sequences retrieved form independent integrants are needed for genome-wide target profiling of piggyBac and Tol2 in the human genome.
Primers were designed based on sequences from Arabidopsis thaliana retrieved from TAIR website and a N. attenuata transcript library (NCBI GEO Database accession number GSE30287).
Third, we selected 7 predicted SPEPs that were retrieved based on sequence similarity to known effectors or ECs from other plant pathogenic fungi (Table 1).
The molecular functional effects of non-synonymous SNPs based on sequence and structure analysis were retrieved from the SNPs3D web resource and database 36 for genes curated in the Comparative Toxicogenomics Database (CTD) 35 to have a relationship with arsenic.
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