Sentence examples for based on sequences published from inspiring English sources
The NR2A, NR2B, NR2C, NR2D, NR3A, and GAPDH mRNA transcripts were quantified using the oligonucleotide primers for real-time PCR amplification designed based on sequences published in the Harvard Primer Bank and the NCBI primer-designing tool where the sequences were blasted.
Thereby, SNP selection was based on sequences published in the GenBank database [accession nos.
The filaggrin gene was amplified by polymerase chain reaction (PCR) based on sequences published by Smith et al. [ 6].
Of the microsatellite markers designed, ten, all based on sequences published by the Leishmania Genome Project [ 11], have proved to be suitable for multilocus microsatellite typing (MLMT).
The tetracycline-regulated pLKO shRNA vector has been described previously [ 16], and was used to generate tet-pLKO NFAT1 shRNA, based on sequences published previously [ 14].
Primers were designed for the desired regions in CGs CRY1 (fragment designation: CRY1-b1), CRY2 (fragment designation: CRY2-2), LHY (fragment designation: LHY-4), GI, HD6, and SbD8 [ 66] based on sequences published in public databases (NCBI and Phytozome) using Primer Premier Software (Premier Biosoft International, Palo Alto, CA, USA).
Intergenic region primers were designed based on sequence published previously.
Primers for PAQR genes were based on sequences from published goldfish sequences (indicated above).
Two antisense oligonucleotides were synthesised (ACGT, Toronto, Ontario, Canada) based on sequences recently published by Silber et al (2002).
New pHMMs were made for discovery of terpene synthases based on the sequences published in [ 20], lanthipeptides based on the required cyclase domain, see [ 21] for review, and thiazole-oxazole modified microcins, or TOMMs based on the YcaO domain [ 22].
Based on the sequences published in GenBank and Ensembl, we designed specific primers and detected by PCR three mRNA species in murine tissues and eight variants in human cells.
