Sentence examples for based on sequences obtained from inspiring English sources

For use in quantitative PCR (qPCR), we developed archaeal-specific amoA primers based on sequences obtained from our clone library work (those known to be present in our BSC samples) as well as those available in public databases.

The new PCR assay was based on sequences obtained after randomly amplified polymorphic DNA (RAPD)- PCR analysis of a group of isolates related to diving that had been identified previously by pulsed-field gel electrophoresis (PFGE).

Based on sequences obtained, specific primers were designed to amplify the regions cox2-rrnS, cob-cox3 and cox3-cox1.

BuT5 and P-element primers BL, BR, P3, and P13 were designed based on sequences obtained by bioinformatic searches (from D. buzzatii and D. mojavensis).

Although all 201 primer pairs were designed based on sequences obtained from a leafy spurge EST-database (for details, see M & M), the possibility exists for different paralogues and alleles of target genes being amplified by a given primer pair.

Primer pairs (20 24 nucleotides) were designed using Lasergene (DNASTAR, Inc., Madison, WI) sequence analysis software from 201 clones annotated to genes based on sequences obtained from a leafy spurge EST-database [ 36].

Polymerase chain reaction primer pairs for mRNA quantification, intending to flank at least one intron, were designed based on coding sequences obtained from the GenBank Sequence Database (http://www.ncbi.nlm.nih.gov/Genbank/index.html) (Table 1>).

A combination of subtype designations based on sequence data obtained from two or more gene loci.

These GSPs were designed based on sequence data obtained from 1.37 kb EST sequence.

Primers were designed based on sequence information obtained from the Phytozome (v. 9.1) [ 24].

Oligonucleotides for CSGE and sequencing were designed using the Primer3 software based on sequence information obtained from public databases (Genomic sequence NC_000017.9, mRNA NM_012138.3).