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In agreement with this model of protein-crystal interactions, we have synthesized highly anionic peptides based on sequences in OPN and shown that these are both unordered and potent inhibitors of HA growth.
In-gel tryptic digest of the protein bands (33 kDa, 28 kDa, 27 kDa and 8 kDa) and analysis by MALDI-TOF spectrometry identified the tryptic fragments as human enterovirus 71 viral proteins based on sequences in NCBI Entrez.
We note that the start methionine remains the same in all Dync1i2 transcripts as this lies in exon 2. To survey Dync1i2 splicing we designed two RT-PCRs based on sequences in the UCSC Genome Browser (exon 1a: uc008kai.1, uc008kai.1; exon 1b: uc008kaj.1, uc008kaj.1).
A "universal" primer set based on sequences in the phosphoprotein (P) gene that are highly conserved across the morbillivirus genus and that should amplify copy DNA from all known morbilliviruses.
It would be interesting to see what the general distribution of this gene is based on sequences in publicly available metagenomic datasets.
GeneXpert detects methicillin resistance or susceptibility (MRS A/MSSA based on sequences in staphylococcal protein A (spa), SCCmec and mecA genes and has a turnaround time of 1 hour.
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The ranking from longest to shortest based on sequencing in the peach reference genome sequence is: scaffold 1, scaffold 4, scaffold 6, scaffold 2, scaffold 7, scaffold 3, scaffold 8 and scaffold 5.
The pioneering so-called 2G NGS platforms available at the time of writing are based on sequencing, in parallel, vast numbers of genome fragments sheared into lengths of approximately 35 400 bp.
The primers employed in the current study were based on sequences available in GenBank and designed by using the Primer3 (version 4.0.0) software (http://primer3.wi.mit.edu).edu
Thereby, SNP selection was based on sequences published in the GenBank database [accession nos.
The design of oligonucleotide primers specific for the different targets was based on sequences available in the Genbank.
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