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Genetic analyses and phylogenetic reconstruction were based on sequences flanked by primers 1902V-LCM and 2346C-LCM.
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OsDR10 was isolated from Minghui 63 by PCR amplification using primers 87G8-F2 (5'-CAGAATTCAACTTTTATCACACGTTTAACG-3') and 87G8-R3 (5'-ACGGATCCTTCATCATCGTCATCCTC-3') designed based on the sequences flanking OsDR10.
To exploit these polymorphisms in the development of molecular markers, we designed primer pairs based on genomic sequences flanking carefully selected mPing insertions (Fig. 2A).
Based on the sequences flanking each of the EPIYA motifs, four distinct EPIYA segments (EPIYA-A to -D) are defined (Fig. 1A) (Hatakeyama, 2004).
Locus specific primers were designed based on unique sequences flanking gene PGSC0003DMG400010146, which were used to generate and sequence an amplicon from the individuals of the CHIPS-ALL population.
Three primers were designed based on the sequences flanking the insertion in the third exon of the sorghum GBSS I gene: non- waxy-specific (Wxonly), waxy-specific (wx a ) and non-specific (Wxcomn).
For the NL map, we assigned 6877 SNPs to putative great tit chromosomes based on sequence homology between SNP flanking sequences and the zebra finch genome sequence.
Thus the proteins from each organelle set were redundancy reduced by using BLASTClust to cluster them based on sequence similarity in their TMD and flanking sequences, and then we removed any with greater than 30% identity over this region (Altschul et al., 1997).
Primers flanking mPing insertion sites in EG4 were designed based on sequence information from a previous study.
Primers were designed based on flanking sequences of SSRs using the Primer 3 program (http://primer3.sourceforge.net/).net/
ABACAS automatically extracts gaps on the pseudomolecule and, based on flanking sequences above a base quality threshold, designs primers for gap closure using Primer3 (Koressaar and Remm 2007).
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