Sentence examples for based on sequences available from inspiring English sources
Primers were designed using Primer3Plus (http://primer3plus.com/) and Oligo Calc (http://www.basic.northwestern.edu/biotools/oligocalc.html) based on sequences available on GenBank from A. baerii for hsp70 (no. HM348777.1) and from sequences of A. schrenckii (no. JX477807.1) and A. ruthenus (no. JN700181.1) for hsp90.
PCR primers were based on sequences available at GenBank (AB076001– and23991) and were designed to amplify the non-coding regions of TTSuV1 and TTSuV2.
Gene-specific primers for rat Nav channel isoforms, α-actin and GAPDH were designed based on sequences available through PubMed (for sequences see Table 1).
Forward and reverse primers (Table 1) for the aforementioned genes were designed based on sequences available in GenBank (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi) using the MIT Primer 3 designer software (http://wi.mit.edu/cgi-bin/primer3/primer3_www.cgi), and were confirmed for specificity using the basic local alignment search tool (www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/
The design of oligonucleotide primers specific for the different targets was based on sequences available in the Genbank.
The primers employed in the current study were based on sequences available in GenBank and designed by using the Primer3 (version 4.0.0) software (http://primer3.wi.mit.edu).edu
The β-1, 4-endoglucanase gene of IARI-SP-1 was amplified using degenerate primers designed based on sequences already available in NCBI GenBank.
Based on sequences more recently available, we found that this primer has 3 mismatches with some Prasinophyceae and Mamiellophyceae, as well as 4 mismatches with Prymnesiophyceae (Figure S1).
It would be interesting to see what the general distribution of this gene is based on sequences in publicly available metagenomic datasets.
