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We did not filter out mutation mentions based on sequence validation, so we consider all the extracted mutations from EMU.
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In addition, to take into account non-CDSs that cannot be used in dS approaches, we developed a new validation procedure based on sequence conservation, which can be applied to both coding and non-CDSs.
SGP-1: prediction and validation of homologous genes based on sequence alignments.
Once the ANN was trained, we tested the sensitivity and accuracy of the model to identify specific outcomes based on sequences presented as validation samples that were not used in the training process.
As an alternative method of cross validation, we considered the division of the dataset of 137 mutants into various subsets or folds based on sequence similarity.
Design and validation of siRNAs are based on sequence-dependent analysis (so-called "on-target analysis").
The discrepancy between SNP detection and validation could also be the result of preferential allele expression or allele silencing in the pooled cDNA sample that was used because the initial SNP identification was based on cDNA sequencing whereas validation was made using PCR fragments amplified from genomic DNA.
Using these bounds, in the second part of the paper, we derive a sequential scheme, based on a sequence of optimization and validation steps.
While this dataset had only two MRI sessions, rather than 10, this dataset used an anatomical MRI sequence that was optimized for brain morphology research (based on prior validation work assessing cortical thickness and subcortical volume) [7, 68].
The results of deep sequencing correlated well with those of qPCR in our study based on the validation results of randomly selected miRNA and mRNA genes.
In addition, sample size calculation strategy based on these validation methods is lacking.
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