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Although this could indicate some systematic bias in peak calling, Kharchenko et al. argue that, based on sequence tag distributions, these sites are likely bound by NRSF under the ChIP-seq experimental conditions (see Supplementary Fig. 9 from Kharchenko et al. [31]).
Peaks were ranked based on sequence tag counts.
These include the widely-used microarray as well as the more recently developed methods based on sequence tag abundances such as the Massively Parallel Signature Sequencing (MPSS) technology.
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The library was subsequently sub-divided in 4 parts based on sequence tags added during library construction.
For example, PDS search can be successfully combined with de novo sequencing algorithm for filtering databases based on sequence tags as it was implemented in the InsPecT project [14].
As the use of high-throughput sequencing becomes more prevalent, there is a growing need for a method to efficiently identify statistically significant genomic features based on sequence tags.
Therefore, a reliable protocol of tag-based gene expression profiling based on sequencing of longer tag fragments is highly desirable.
For InDels and SNPs calling, the trimmed reads were clustered into RAD-tags based on sequence similarity using Stacks under default parameters [ 51].
The 82-bp first reads within each individual plant were grouped into tag reads based on sequence similarity by allowing a maximum of two mismatches between any two RAD reads.
Mostly, Gα-, Gβ- and Gγ-subunit gene sequences in Pinaceae are predicted based on expressed sequence tag (EST) sequences [ 9, 22].
Here we report a transcript sequencing approach based on Expressed Sequence Tag (EST) analysis of Hpa-infected Arabidopsis leaves.
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