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Objectives: Here we report a new real-time (LightCycler) quantitative PCR for the detection of HBV DNA based on sequence specific hybridisation probes (designed in-house), targeting the HBV surface antigen.
Unfortunately such a Polymerase Chain Reaction (PCR) approach based on sequence specific primers was not suitable for TaSK2-C as this sequence did not have an appropriate specific nucleotide insertion or deletion that distinguished it from the other ones.
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Resulting centroid sequences were used for downstream phylogenetic analyses based on sequences specific to the lemuriform families Cheirogaleidae (the dwarf and mouse lemurs) and Lemuridae (the true lemurs).
For FOSL-1 and FHL2 siRNA transfection, siRNA was designed based on sequences specific for human cDNA; 5'-GCA TCA ACA CCA TGA GTG G-3' for FOSL-1 and 5'-GCA AGG ACT TGT CCT ACA A-3' for FHL2.
Genome editing based on sequence-specific designer nucleases, also known as programmable nucleases, seeks to modify in a targeted and precise manner the genetic information content of living cells.
It is critical for diagnostic chips based on sequence-specific probes to eliminate such non-specific hybridisation.
Hek2 might therefore be one factor that helps discriminate mRNAs and "mRNA-like" lncRNAs from "standard" lncRNAs, based on sequence-specific binding.
The MLPA method is based on sequence-specific probe hybridization to genomic DNA, followed by PCR amplification of the hybridized probe (with one FAM-labeled primer) and semi-quantitative analysis of the PCR products.
These tools are based on sequence-specific binding of a nuclease that cleaves the genomic double-stranded DNA (dsDNA), thereby inducing a double strand break (DSB) (Gaj et al. 2013).
With respect to a new subgroup classification for the P2Y subfamily based on sequence similarity, specific ligand-binding motifs, and gene clustering, we propose that GPR99 is a receptor for nucleotide ligands.
Although these Myb TFs were suggested to be involved in the lupulin metabolome production based on sequence similarity and specific expression in hop cones [ 19, 20], their influence on the activation of chs genes has not been investigated in detail.
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