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We developed a simple and rapid DNA-based molecular identification system for the Lemnaceae based on sequence polymorphisms.
A set of primers and hydrolysis probe were designed based on sequence polymorphisms in the large ribosomal RNA subunit (28S).
Eighteen genes within the QTL region on chromosome 6 were selected based on sequence polymorphisms with G. soja accession IT182932 (Tables 7 and 8 ).
In the past two decades, it has become possible to construct high-density maps for almost all areas of chromosomes using DNA markers based on sequence polymorphisms.
As shown in Figure 4A, the number of CCHC arrays in NC varies depending on the Ty3/Gypsy lineage and the Retroviridae class (links in red), and both the flap and the GPY/F motifs delineate multiple variant motifs based on sequence polymorphisms preserved similarly (links in black and blue, respectively).
Our assay differs from conventional multiplex PCRs, which often target the invasion plasmid H (ipaH) gene, O antigen synthesis genes, and the 16S rRNA gene for detection of Shigella spp. [ 18– 20]; in those cases, the diagnosis is often based on sequence polymorphisms or differences rather than on the absence or presence of a gene.
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Fully-automated detection system for manipulation of bloom-forming genera of cyanobacteria, Microcystis spp., was developed using a specific DNA probe designed based on sequence polymorphism within the 16S ribosomal DNA (rDNA) of this strain.
A recent origin is consistent with our estimates of the minimum age of the 2Rj inversion based on sequence polymorphism near a breakpoint (∼0.4Ne generations) and the lack of fixed differences between alternative arrangements.
Based on sequence polymorphism, either CAPs or SNP diagnostic markers were developed.
Candidate genes identified based on sequence polymorphism generally differed from those identified based on variation in gene expression among lines.
The phylogenetic tree exclusively based on rearrangement and duplication events is congruent with the tree based on sequence polymorphism, validating our evolution model.
More suggestions(11)
based on restriction polymorphisms
based on deletion polymorphisms
based on sequence networks
based on chromosomal polymorphisms
based on sequence alignments
based on sequence homologies
based on length polymorphisms
based on sequence features
based on gene polymorphisms
based on sequence similarities
based on insertion polymorphisms
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