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LincRNAs clustered at the same locus could not be assumed to be isoforms due to different tissue assemblies, and hence we selected one lincRNA from each locus based on sequence length and consensus alignment with the genome sequence.
The examination of ITS nucleotide variation also revealed the possible division into three distinct groups based on sequence length among the 91 isolates of P. noxius from Taiwan (Table 4).
The best cluster representative was identified based on sequence length and average quality values.
Indels are frequently binned into the categories of "small" and "large" based on sequence length.
Densities of microsatellites were based on sequence length excluding segmental duplications and repeats, unless stated otherwise.
The data was then processed using SeqClean [ 46] using standard parameters but without any filtering based on sequence length.
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The key feature of using a hybridisation-based end point with MAPH is that discrimination of amplified probes is based on sequence and not fragment length.
Sequences were attributed to putative genes based on sequence identity and intron length.
Next, we predicted multicopper oxidase-encoding genes based on sequence similarities, and full-length multicopper oxidase-encoding genes were curated using the GeneWise algorithm.
A schematic representation of all the family members, based on sequence alignments with the full length functional gene (Additional file 3), is included in Figure 3.
ESTs were clustered using Malign, a kmer based alignment tool that clusters ESTs based on sequence overlap (kmer = 16, seed length requirement = 32 alignment ID ≥98%).
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based on price length
based on sequence information
based on sequence tag
based on sequence homology
based on sequence mining
based on sequence selection
based on trip length
based on sequence complementarity
based on sequence solubility
based on sequence content
based on dendrogram length
based on sequence conservation
based on sequence comparison
based on sequence divergence
based on chromosome length
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