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The extremely low rate of novel taxa is due to the stringent algorithm involved in the matching criteria based on sequence coverage (reference-based) and de novo assembly of Illumina shotgun reads (reference independent), which eliminates nearly all sequence errors.
Variants were filtered based on sequence coverage and quality scores.
The cn.MOPS approach is also based on sequence coverage data partitioned into non-overlapping windows.
The copy number estimates reported here are based on sequence coverage and are in most cases reliable.
Primers (Additional file 1) were designed based on sequence coverage and loci were selected that were single copy in the wRi genome.
The data set was further modified to include additional family members based on sequence coverage and to exclude family members with internal stop codons and matches to TEs.
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Developed measure of gene regulation was able to differentiate these two sets based on sequencing coverage near gene transcription start site.
Depth of coverage methods do not require breakpoint capture or have specific size restrictions, yet such methods are hampered by low signal to noise ratio due to sparse sampling (a product of low overall coverage) and sampling bias based on sequence GC-content, bait coverage, and other factors affecting capture efficiency.
The template was the structure of δ-opioid receptor bound to naltrindole (PDB accession code: 4EJ4) which was selected based on sequence identity, similarity, and coverage as suggested by the server (no user intervention).
All the remaining sequences were clustered based on sequence similarity using BLASTCLUST program with 90% identity and 80% coverage.
STS data were analyzed by at least two independent scientists and the final consensus haplotypes were based on redundant sequence coverage over all positions (at least two independent sequence reads).
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based on sequence variation
based on sequence selection
based on city coverage
based on sequence solubility
based on sequence content
based on imputation coverage
based on signal coverage
based on news coverage
based on population coverage
based on insurance coverage
based on sequence analysis
based on sensing coverage
based on sequence conservation
based on sequence similarity
based on sequence comparison
based on sequence divergence
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