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Moreover the TargetScan Human resource, which provide miRNA target predictions based on sequence complementary to target sites with emphasis on perfect base-pairing in the seed region and sequence conservation [ 35], was used.
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The resulting imperfect miRNA:miRNA* duplex is then unwound and one strand is selected as the mature miRNA, based on sequence thermodynamic properties [25], whereas the complementary passenger strand is usually subjected to degradation.
A microarray based on oligonucleotides complementary to individual MAPH probes would allow detection and quantification based on sequence rather than on size.
Different from those constructions using complementary sequences as a kernel, the proposed constructions are based on sequences with periodic and aperiodic zero-correlation zone (PAZCZ).
Interestingly, based on sequencing the same sample, Clark et al. demonstrated that exome and whole genome sequencing are complementary.
Primers (Additional file 12) complementary to the genes were designed based on the assembled P. cactorum unigene sequences.
The MGA also has an adaptable the RBS model based on complementary sequences of the 3′ tail of 16S ribosomal RNA, and precisely predicts translation starts of genes even when input genomic sequences are short and anonymous sequences.
The ligation probes and universal linkers were phosphorylated and then ligated based on the sequence specificity complementary to the genomic DNA template.
The numbering is based on the complementary DNA sequence, with +1 corresponding to the A of the ATG translation initiation codon.
Genetic typing was based on nucleotide sequence differences in complementary DNA (cDNA) for the nucleoprotein gene as aligned with sequence for the Pasteur vaccine strain of rabies virus (7 ).
C/D box snoRNAs direct 2'-O methylation, and are relatively easy to identify based on conserved sequence elements and complementary binding to the target RNAs.
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