Sentence examples for based on sequence available from inspiring English sources

$Primers were designed based on sequence available in GenBank.

The MR1-1 scFv was artificially synthesized based on sequence available from Genbank (accession no. U76382).

Primers were designed using Primer3Plus (http://primer3plus.com/) and Oligo Calc (http://www.basic.northwestern.edu/biotools/oligocalc.html) based on sequences available on GenBank from A. baerii for hsp70 (no. HM348777.1) and from sequences of A. schrenckii (no. JX477807.1) and A. ruthenus (no. JN700181.1) for hsp90.

Gene-specific primers for rat Nav channel isoforms, α-actin and GAPDH were designed based on sequences available through PubMed (for sequences see Table 1).

PCR primers were based on sequences available at GenBank (AB076001– and23991) and were designed to amplify the non-coding regions of TTSuV1 and TTSuV2.

Forward and reverse primers (Table 1) for the aforementioned genes were designed based on sequences available in GenBank (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi) using the MIT Primer 3 designer software (http://wi.mit.edu/cgi-bin/primer3/primer3_www.cgi), and were confirmed for specificity using the basic local alignment search tool (www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/

The design of oligonucleotide primers specific for the different targets was based on sequences available in the Genbank.

Primer sequences for the microsatellite markers were based on sequences available from The Jackson Laboratory and purchased from Integrated DNA Technologies IDTT, Coralville, IA, USA).

PCR and extension primers were designed on the Sequenom Assay Designer 3.1 Software (San Diego, CA) based on sequences available through the National Center for Biotechnology Information (dbSNP; http://www.ncbi.nlm.nih.gov/projects/SNP)).nih.gov/projects/SNP

The primers employed in the current study were based on sequences available in GenBank and designed by using the Primer3 (version 4.0.0) software (http://primer3.wi.mit.edu).edu

Exact match PCR primers to obtain Entamoeba genes for independent sequencing were designed based on sequences available from the website of the ongoing genome projects [ 67] that showed similarity to the studied genes.