This phylogeny-based method is expected to complement our approaches based on sequence alignments and structural analysis.
Taxonomy-dependent methods are based on sequence alignments, phylogenetic models and/or oligonucleotide patterns.
The assembly is based on sequence alignments driven by identical regions shared by the fragments (Table 2).
Traditional division of the dynamin subunit into GTPase domain, middle domain and GTPase effector domain based on sequence alignments and biochemistry is not supported by recent structural data.
Based on sequence alignments and structural studies, three single amino acid substitutions, D500G, D500E, D500S and a glycine insertion, are introduced using site-directed mutagenesis to evaluate the role of Asp500 located in the C-terminal segment close to the T1 copper center.
Based on sequence alignments and homology modeling, four cysteines are potentially available for labelling in hERG.
The conservation scores were generated based on sequence alignments of 16 vertebrate genomes with the human genome.
Design based on sequence alignments and computer modelling resulted in a humanized version bearing 13 mutations compared to initial murine scFv.
Based on sequence alignments, Exon 10 is readily identified in mammals and lizard, but not in chicken, frog (X. tropicalis) or fishes (Figure 1A).
The identification of possible contamination based on sequence alignments, however, seems to provide the only reliable option currently available to classify single sequences as contamination.
