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Although the two-moment bulk scheme also considers the breakup of cloud droplets, this difference is minor based on sensitivity experiments examining the breakup process (figure not shown).
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To determine the identifiability of the resulting model an identifiability assessment was carried out, based on sensitivity analysis and optimal experiment design criteria.
Furthermore, a discretization method based on sensitivity functions is proposed.
The Δt for cloud microphysics is determined as the largest time step to avoid artificial noise, and the ratio of Δt for dynamics to Δt for other physics is set to 10 based on the sensitivity experiments (see Appendix 2 of this paper for details of the sensitivity experiment).
It is often of interest to estimate parameters of a response curve based on sequential sensitivity experiments.
The time step for physics (except for cloud microphysics) was set as 10 × Δt dyn based on the sensitivity experiment (Nishizawa et al., 2015).
The customized SPEA2 algorithm is further used to solve the problem, and some managerial insights are provided based on experiments and sensitivity analysis with the model.
Blasticidin (Invitrogen) was added in appropriate samples to select for transduced cells the day after virus removal, at a concentration of 8 μg/ml, based on preliminary experiments of AF cell sensitivity to blasticidin.
In particular, drug sensitivity testing on primary cells is often based on dose response experiments, which pose a more stringent requirement for data quality and for intra- and inter-plate variation.
A formal sensitivity analysis based on design of experiments shows that on critical interfacial stresses, interphase to film thickness ratio and film to substrate Young's moduli ratio has the most impact, while type of elastic moduli variation in the interphase and indentor width to film thickness ratio has the least impact.
Based on our experiments, ASS1 is a putative negative regulator of TRAIL sensitivity, and LOF induced an increase in caspase-3/7 activation.
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