Sentence examples for based on seed sequence from inspiring English sources
We then grouped all 415 zebrafish miRNAs into families based on seed sequence identity as a means to identify possible functional redundancy.
The mature miRNA with RISC will bind to and silence its target mRNA based on seed sequence complementarity, generally at the 3' UTR.
(XLSX 259 kb) Additional file 4: Identification of Novel Bovine miRNAs Based on Seed Sequence Alignments (A) and Sequence Identity of Novel Bovine miRNAs from BLAST Analysis of miRBase 19 (B).
For those miRNAs with less or no validated targets, target mRNAs were predicted based on "seed sequences" using the TargetScan program [ 19].
Based on seed sequences, 35 miRNA families were identified (26 known and 9 novel miRNA families) to contain at least two members and the identification of novel miRNAs added new members to 5 known families (Table 6).
We used the computational algorithms miRanda, PicTar, and TargetScan to identify prion-related potential target genes of the de-regulated miRNAs based on seed-sequence homology with 3' UTRs of mRNA transcripts [26] [28].
Many miRNAs are clustered into families based on their seed sequence; thus, miRNAs with the same seed sequence may target the same set of genes, providing the possibility of functional redundancy and cooperation among different miRNAs [ 19– 219.
Second, TargetScan (http://www.targetscan.org) was used to detect target gene candidates based on seed complementarity on UTR database 6.0 and our porcine RefSeq transcript with our miRNA seed sequence [ 99].
Discontinuous distribution across the animal kingdom showed diversity of miRNA sequences based on certain seed sequence, and the sufficient variations flank mature miRNAs could contribute to the evolutionary diversification of these key regulatory genes [28].
based on seed values dependent on a random number generator.
Finally, miRDeep assigned a score to each read based on: (a) seed sequence homology to known human miRNA, (b) presence of a star sequence, (c) minimum free energy (RNAfold), (d) energetic stability (Randfold), (e) frequency of reads that correspond to Dicer processing.
