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The first is the Battacharyya bound [35], which is based on scatter matrices.
Separability measures based on scatter matrices are normally preferable to probabilistic distances because they do not require an estimation of the probability density function of the classes or that those probability density functions are known a priori.
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Dead cells were excluded based on scatter profile.
First, the risk factors were categorized, using cut offs based on scatter plots.
Cells were gated based on scatter properties.
Neutrophils were gated based on scatter properties.
The good arrays were selected based on scatter plot analysis.
Inflammatory cell populations (monocytes, neutrophils, eosinophils, and lymphocytes) in blood were identified and gated based on light scatter properties (forward scatter and side scatter).
Live cells were gated based on forward scatter and side scatter and/or by propidium iodide dye exclusion.
DC were gated based on side scatter and forward scatter followed by doublet discrimination gating (Fig. 1A).
Figure 2a shows a representative histogram plot based on forward scatter and side scatter gated cells.
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