To avoid selection bias, the set of significant features were re-selected based on samples only in the training set in each cross-validation round.
Technical variation was assessed by calculating the coefficient of variation for each antibody based on samples measured in triplicates.
The present analysis was based on samples collected in 15 global GSK sponsored clinical studies, 14 of which involved subjects with respiratory disease.
Most studies that have examined the etiology of GDM have been hospital based or have been based on samples of births in a particular region (4, 5).
They were instigated by studies from Sweden based on samples of individuals born in the town of Överkalix.
The manufacturer's intra-assay coefficient of variation (CV) based on samples assayed 12 times was ≤12.3%, and interassay CV based on samples assayed in duplicate in 11 different series was <14.2%.
Based on sample collections in a full scale production environment two cases of cleaning monitoring by UV are evaluated.
Therefore, we calculated weights based on sample sizes in such a way that the group (or groups) with the largest sample size were given a weight of 1 (adapted from [31] page 42).
These data gave us a sample size of 17 patients; however, because we were unsure of the variability of the data, and based on sample sizes in previous studies, we tripled our sample size.
This study is based on one sample in a specific geographical area.
